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Author:
Date:
2014-05-05
[Abstract] A biofilm is a multicellular consortium of surface associated microbes surrounded by a hydrated, extracellular polymer matrix. The biofilm matrix plays a critical role in preventing desiccation, acquiring nutrients, and provides community protection from environmental assaults. Importantly, biofilms are significantly more resistant to antimicrobials relative to their free-swimming counterparts. The level of antimicrobial tolerance is influenced by a number of factors, including genetic/adaptive resistance mechanisms, stage of biofilm development, and pharmacokinetics of the antibiotic. Here, ...
[摘要] 生物膜是由水合的细胞外聚合物基质包围的表面相关微生物的多细胞聚生体。生物膜基质在防止干燥，获得营养物质和提供社区保护免受环境攻击中起关键作用。重要的是，相对于其自由游泳的对应物，生物膜显着地更抗抗微生物剂。抗微生物耐受性的水平受许多因素影响，包括遗传/适应性抗性机制，生物膜发育阶段和抗生素的药代动力学。在这里，我们描述了一种基于体外微量滴定的测定法，用于定量短曝光时间（2小时）的生物膜（MBC-B）的最小杀菌浓度。该暴露时间显着短于传统方案中描述的标准过夜和24小时治疗。开发该测定法以接近抗生素在其被代谢，被宿主蛋白螯合或消化之前在一次性治疗期间可用的时间。
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Author:
Date:
2012-11-05
[Abstract] This is a general protocol for the isolation and maintenance of primary MPM cultures as a tool for the identification of Tumor Initiating Cells and early progenitor-targeting drugs (Cioce et al., 2010). Primary cultures can be propagated efficiently for 8-12 weeks and xenotransplanted in NOD/SCID mice while retaining the histofeatures of the originating tumor (Canino et al., 2012). The protocol is suitable for both MPM solid specimens and pleural effusion. For increased clarity, initially two separate sections addressing the isolation of MPM cells from solid tumors and ...
[摘要] 这是用于分离和维持初级MPM培养物的一般方案，作为鉴定肿瘤启动细胞和早期祖细胞靶向药物的工具（Cioce等人，2010）。 原代培养物可以有效繁殖8-12周，并且在NOD/SCID小鼠中异种移植，同时保留起源肿瘤的组织特征（Canino等人，2012）。 该方案适用于MPM固体标本和胸腔积液。 为了增加清晰度，最初提供了两个分开的部分，其涉及从实体瘤和胸腔积液中分离MPM细胞。
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