Penicillin-Streptomycin (10,000 U/mL)

10 ML KARYOMAX COLCEMID溶液

公司名称: Thermo Fisher Scientific

产品编号: 15212012

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Microscopic Detection of DNA Synthesis in Early Mitosis at Repetitive lacO Sequences in Human Cells

Kazumasa Yoshida, Riko Ishimoto and Masatoshi Fujita

Published online: 2022-09-05

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Immunostaining of Formaldehyde-fixed Metaphase Chromosome from Untreated and Aphidicolin-treated DT40 Cells

Author:

Vibe H. Oestergaard,

Date:

2017-05-05

[Abstract] During mitosis chromosomes are condensed into dense X-shaped structures that allow for microscopic determination of karyotype as well as inspection of chromosome morphology.

This protocol describes a method to perform immunostaining of formaldehyde-fixed metaphase chromosomes from the avian cell line DT40. It was developed to characterize the localization of YFP-tagged TopBP1 on mitotic chromosomes and specifically determine the percentage of TopBP1 foci that formed on breaks/gaps as well as ends of individual metaphase macrochromosomes (Pedersen et al., 2015). For this ... [摘要] 在有丝分裂期间，染色体被浓缩成致密X型结构，允许显微检测染色体核型和检查染色体形态。
该方案描述了从禽细胞系DT40进行甲醛固定的中期染色体的免疫染色的方法。它被开发用于表征YFP标记的TopBP1在有丝分裂染色体上的定位，并且具体确定形成于断裂/间隙以及单个中期大染色体末端的TopBP1灶的百分比（Pedersen等，2015）。为此，应用YFP的免疫染色。然而，该协议可以针对其他细胞系或表位进行优化。
【背景】染色中期染色体的显微镜分析是经典的细胞遗传学技术，广泛用于研究和诊断。基本原理包括通过锭子不稳定试剂如谷氨酸诱导中期细胞周期停滞，这将触发主轴装配检查点，从而阻止细胞在中期。这用于富集浓缩染色体的细胞。随后将细胞在低渗溶液中进行溶胀，然后在显微镜载玻片上铺展有丝分裂细胞。最终结果是来自单细胞的显微镜检测染色体，便于染色体核型分析以及个体染色体的研究。传统上，肿胀的细胞用甲醇和乙酸（3:1）固定，然后铺展在载玻片上（Hungerford，1965; Ronne等，1979）。
这里描述的方法使用甲醛而不是甲醇进行固定。这可以用于随后用与甲醇固定不相容的抗体染色。该方案针对鸡DT40细胞的中期传播进行了优化，YFP标记的TopBP1在中期大染色体上的免疫染色（Pedersen等，2015）。 ...

Karyotype Analysis

Author:

Chun-Hung Chou and Muh-Hwa Yang,

Date:

2014-05-20

[Abstract] A chromosome is the structure that organizes DNA and protein in cells. It is a single piece of coiled DNA containing coding and non-coding sequences. Human cells have 23 pairs of chromosomes including 22 pairs of autosomes and one pair of sex chromosome, giving a total of 46 per cell. In tumor cells, chromosomal instability has been considered to be one of the hallmarks of tumor formation. Here we use the karyotype analysis to separate the chromosomes and observe the chromosomes in tumor cells with a microscope.
[摘要] 染色体是组织细胞中的DNA和蛋白质的结构。它是含有编码和非编码序列的单片卷曲DNA。人类细胞具有23对染色体，包括22对常染色体和一对性染色体，每个细胞总共46个染色体。在肿瘤细胞中，染色体不稳定性已被认为是肿瘤形成的标志之一。这里我们使用核型分析来分离染色体，并用显微镜观察肿瘤细胞中的染色体

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Penicillin-Streptomycin (10,000 U/mL)

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