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PE anti-mouse CD3ε Antibody

PE抗小鼠CD3ε抗体

公司名称: BioLegend
产品编号: 100307
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Competitive Bone-marrow Transplantations
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Date:
2014-06-05
[Abstract]  Competitive bone marrow transplantation assay measures multi-lineage reconstitution of hematopoiesis in irradiated transplant recipient mice. Thus this assay is routinely used to determine haematopoietic stem and progenitor cells (HSPCs) functionality in vivo. The principle of the method is to transplant bone marrow donor cells (derived from transgenic mice of choice) on C57BL6 background together with normal competitor bone marrow. In order to distinguish donor from competitor cells upon transplantation, usually competitor mice are congenic and carry the differential B cell antigen ... [摘要]  竞争性骨髓移植测定测量照射的移植受体小鼠中造血的多谱系重建。 因此,该测定常规用于在体内确定造血干细胞和祖细胞(HSPC)功能。 该方法的原理是将骨髓供体细胞(源自选择的转基因小鼠)与正常竞争剂骨髓一起移植到C57BL6背景上。 为了在移植时区分供体和竞争者细胞,通常竞争性小鼠是同基因的并携带最初称为Ly5.1和CD45.1的差别B细胞抗原。典型的竞争性骨髓移植实验将含有两个移植组, 供体(选择的转基因小鼠及其对照)与正常竞争剂竞争移植,并在血液和骨髓中评价移入效率。

In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse
Author:
Date:
2012-12-20
[Abstract]  Antigen cross presentation is important for effective immune responses to tumors and viral infections. Dendritic cells are professional antigen presenting cells and are unique in their ability to cross-present exogenous antigens on MHC class I molecules and activate antigen specific cytotoxic T cells. This protocol describes antigen cross presentation by dendritic cells (DCs) (bone marrow derived DCs and splenic DCs) in an in vitro and in an in vivo assay system using soluble ovalbumin protein. [摘要]  抗原交叉呈递对于对肿瘤和病毒感染的有效免疫应答是重要的。 树突状细胞是专业抗原呈递细胞,并且在其将MHC I类分子上的外源抗原交叉呈递并激活抗原特异性细胞毒性T细胞的能力方面是独特的。 该方案描述了在体外和在体内使用可溶性卵白蛋白蛋白质的体系中通过树突细胞(DC)(骨髓来源的DC和脾DC)的抗原交叉呈递 。

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