| Xanthoferrin Siderophore Estimation from the Cell-free Culture Supernatants of Different Xanthomonas Strains by HPLC
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Author:
Date:
2017-07-20
[Abstract] Xanthomonads can scavenge iron from the extracellular environment by secreting the siderophores, which are synthesized by the proteins encoded by xss (Xanthomonas siderophore synthesis) gene cluster. The siderophore production varies among xanthomonads in response to a limited supply of iron where Xanthomonas campestris pv. campestris (Xcc) produces less siderophores than Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc). Siderophore production can be measured by HPLC and with the CAS (Chrome azurol ...
[摘要] 黄单胞菌属可以通过分泌铁载体来从细胞外环境中清除铁，这些铁载体由由xss 编码的蛋白质（anthomonas 基因簇。 由于Xanthomonas campestris pv的铁供应有限，铁载体生产因黄托曼德而异。 野蓟菊（Xcc）产生比黄单胞菌（Xanthomonas oryzae）pv更少的铁载体。 米曲霉（Xoo）和黄单胞菌Xanthomonas oryzae pv。 oryzicola（Xoc）。 铁载体生产可以通过HPLC和CAS测量（ Chrome azurol S ）标准板测定，然而HPLC是在Xanthomonads中用于铁氰菊酯定量的CAS-琼脂平板测定的更准确的方法。 在这里我们描述如何使用高效液相色谱来量化黄单胞菌中的铁载体。
【背景】细菌植物病原体 Xanthomonas 具有（α-羟基羧酸盐型铁载体;类似于弧菌铁蛋白）和编码参与铁载体介导的铁吸收的外膜受体（Pandey和Sonti）所需的操纵子，2010; ...
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| Purification and Structural Analysis of QS-inhibiting Compounds from Staphylococcus delphini
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Author:
Date:
2014-06-05
[Abstract] The knowledge that many pathogens rely on cell-to-cell communication mechanisms known as quorum sensing, opens a new disease control strategy: quorum quenching. Here we present a purification protocol for molecules excreted by a group of Gram-positive zoonotic pathogen bacteria, the ‘Staphylococcus intermedius group’, that suppress the quorum sensing signaling and inhibit the growth of a broad spectrum of Gram-negative beta- and gamma-proteobacteria. These compounds were isolated from Staphylococcus delphini (S. delphini). They represent a new class of quorum ...
[摘要] 许多病原体依赖于被称为群体感应的细胞间通信机制的知识，开启了一种新的疾病控制策略：群体猝灭。 在这里，我们提出了一组由革兰氏阳性动物传染病原体细菌分泌的分子的纯化方案，所述细菌是抑制群体感应信号传导并抑制广谱革兰氏菌的生长的'中间体葡萄球菌 - 阴性β-和γ-变形细菌。 这些化合物从葡萄球菌delphini （ S. delphini ）中分离。 它们代表一类新的具有化学式N - [2-（1H-吲哚-3-基）乙基] - 脲和N，N-二 - - （2-苯乙基） - 脲，其分别命名为yayurea A和B. 这些物质可以使用这种可扩大的纯化方法从培养物上清液中分离和纯化
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