| ELISA Detection of Endogenous Serum Albumin in the Mouse Brain: A Measure of Extravasation Following Brain Injury
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Author:
Date:
2015-05-05
[Abstract] After stroke and brain contusion, serum proteins extravasate into nerve tissue through disrupted blood-brain barrier (BBB). Because extravasations of serum proteins result in vasogenic brain edema, serum albumin level in the brain is an indicator of BBB disruption and brain edema after brain insults. In this protocol, extravasation of endogenous albumin is measured in the damaged mouse brain, which would be valuable in the evaluation of vasogenic brain edema formation (Michinaga et al., 2014).
[摘要] 在中风和脑挫伤后，血清蛋白通过破裂的血脑屏障（BBB）外渗到神经组织中。 因为血清蛋白的外渗导致血管性脑水肿，脑中的血清白蛋白水平是脑损伤后BBB破坏和脑水肿的指标。 在该方案中，在损伤的小鼠脑中测量内源性白蛋白的外渗，这在评价血管性脑水肿形成中是有价值的（Michinaga等人，2014）。
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| Thin Sections of Technovit 7100 Resin of Rice Endosperm and Staining
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Author:
Date:
2014-09-20
[Abstract] Starch is a biologically and commercially important carbohydrate that is accumulated in plant storage organs, such as seed endosperm. Starch is water-insoluble and forms transparent grains, referred to as starch grains (SGs). SGs are easily stained by iodine solution and can be observed under a normal microscope. Technovit 7100 resin is suitable for preparation of thin sections from endosperm. The thin sections and iodine staining can visualize SGs clearly inside the endosperm cell. This protocol provides the procedures to prepare thin sections of Technovit 7100 resin from rice endosperm. It ...
[摘要] 淀粉是在植物储存器官（例如种子胚乳）中积累的生物学上和商业上重要的碳水化合物。 淀粉是水不溶性的并且形成称为淀粉颗粒（SGs）的透明颗粒。 SGs容易被碘溶液染色，并且可以在正常显微镜下观察。 Technovit 7100树脂适用于从胚乳制备薄切片。 薄切片和碘染色可以明显地在胚乳细胞内部显现SG。 该协议提供了从水稻胚乳制备Technovit 7100树脂薄片的程序。 它也可以适用于其他植物物种的种子。
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