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Poly-D-lysine hydrobromide

聚-D-赖氨酸氢溴酸盐

公司名称: Sigma-Aldrich
产品编号: P7280
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Isolation, Culture and Differentiation of Adult Hippocampal Precursor Cells
Author:
Date:
2017-11-05
[Abstract]  There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation in a reductionist model. While the neurosphere assay is primarily performed to directly study the proliferative and differentiation potential of cells in individual brains, the monolayer culture allows single cell analysis in a rather homogeneous ... [摘要]  成年哺乳动物脑中有两个神经生态位:侧脑室下脑室区和海马齿状回颗粒下区。 来自这些区域的细胞可以在体外分离和维持,使用两种不同的培养系统评估它们在还原模型中的增殖和分化的潜力。 虽然神经球测定主要是为了直接研究个体脑中细胞的增殖和分化潜能,单层培养允许在相当均匀的细胞群中进行单细胞分析。 在这里,我们描述了两个系统中的神经前体细胞的分离,培养方法和分化。

【背景】在哺乳动物脑中,成人神经干细胞存在于两个主要神经生态位中,即海马齿状回(DG)的下颗粒区(SGZ)和室下区(SVZ)的侧脑室,其允许新生神经元成人的大脑。来自神经生态位的神经前体细胞可以在体外分离和培养以模拟细胞过程,尤其是增殖和分化。两种标准培养系统,贴壁单层培养(Palmer等人,1995; Ray等人,1995)和神经球测定(Reynolds和Weiss,1992和1996 )在20世纪90年代被引入,代表了在体外研究神经祖细胞生物学的有价值的工具。

根据研究问题,每个系统都有其优点和缺点,在选择其中一种或另一种培养方法之前应该仔细考虑。在贴壁单层培养中,细胞生长相当孤立,形成更均匀的培养物。单层允许直接调查和监测单细胞水平的神经前体细胞。受控条件下的形态,增殖和分化等特征可以很容易地分析和可视化。然而,与神经球培养物相比,以单层培养的细胞代表更复杂的模型,因为细胞通常以更少的通常存在于细胞壁中的细胞 ...

Neurosphere Co-culture Assay
Author:
Date:
2016-08-05
[Abstract]  The hippocampal niche is one of two areas in the brain where stem cells reside. In this neurogenic niche, stem cells can be found in close proximity to astrocytes and in contact with microvessels consisting of pericytes and endothelial cells. To study the regulatory interplay of this complex niche network in a simplified in vitro model, we established a co-culture system. We investigate the formation of neurosphere under different co-culture conditions by using primary niche cells. Here, we describe the isolation procedure for primary niche cells culture of astrocytes, endothelial ... [摘要]  真菌形态发生需要细胞壁的修饰和可塑性,这意味着其组分(包括壳多糖和葡聚糖)的合成和重塑。因此几丁质酶和葡聚糖酶活性对于细胞壁生物发生和细胞分裂是至关重要的。几丁质酶活性的定量可能有助于鉴定可能负面影响一些丝状真菌如产生细胞内和分泌型几丁质酶的尖孢镰刀菌的生长和形态发生的结构缺陷。基于它们对壳多糖底物的酶作用将壳聚糖分解酶分类。内酯酶被定义为催化几丁质链中内部点的随机裂解的酶。外切球蛋白酶催化乙酰壳寡糖或N-乙酰葡糖胺从几丁质的非还原端逐步释放,因此分别称为壳聚糖酶和β-N-乙酰氨基葡糖苷酶。在这里,我们描述了一种简单的方法来轻易地纯化几丁质酶,以便比较不同的F的内切几丁质酶活性和外切几丁质酶活性。尖孢镰孢菌株。该方案可适用于任何真菌物种。...

Midbrain Neuron-glia Mixed Cultures
Author:
Date:
2012-04-05
[Abstract]  Mixed neuron-glia cultures provide a unique tool to study cellular contribution and molecular pathways in various neurological disorders. They are also invaluable for exploring neuron-glia interaction under physiological and pathological conditions. The relatively long-lasting midbrain neuron-glia mixed cultures generated following this protocol have been widely used to study the pathogenesis of Parkinson’s disease, the most common neurodegenerative movement disorder. [摘要]  混合神经元-神经胶质细胞培养提供了一个独特的工具来研究细胞的构成和各种神经系统疾病的分子途径。他们在探索生理和病理条件下神经元-胶质细胞相互作用方面大有用处。按照操作指南上相对持久的中脑的神经元—神经胶质细胞混合培养已被广泛用于研究帕金森氏症——最常见的神经性运动障碍疾病的发病机制。这份指南经过了Dr. Hong实验室不同研究人员尤其是Dr. Bin Liu多年的改进。

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