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Author:
Date:
2014-11-05
[Abstract] The purification of a target protein from a complex mixture of proteins is a challenging undertaking. If the target protein has been previously characterised, then information such as subcellular location, function, molecular weight and pI can be used for the design of a purification strategy. However, if the target protein is uncharacterised or little information regarding its characteristics is available, a generic purification protocol can be employed that is optimised as additional characteristics of the target protein are determined during subsequent purification steps. Herein, we ...
[摘要] 从蛋白质的复杂混合物中纯化靶蛋白是一项具有挑战性的任务。 如果靶蛋白以前已被表征，则诸如亚细胞定位，功能，分子量和pI的信息可用于设计纯化策略。 然而，如果目标蛋白质是未表征的或者关于其特征的很少的信息可用，则可以使用通用的纯化方案，其随着在随后的纯化步骤期间测定靶标蛋白质的附加特征而被优化。 在这里，我们描述了从两个革兰氏阴性细菌的联合培养物使用色谱，2D电泳和酶谱的组合纯化和检测聚-γ-D-谷氨酸（PDGA）解聚酶的协议。
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