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Fluo-4

Fluo-4

公司名称: Thermo Fisher Scientific
产品编号: F14201
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Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons
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Date:
2014-12-05
[Abstract]  This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons. [摘要]  该协议包括使用探针SynaptopHluorin的荧光测量的荧光测量的整个过程,所述探针是pH依赖性GFP变体,其荧光在囊泡释放时由于酸性囊泡中的荧光猝灭而在突触上增加。 这种技术提供了一种遗传工具来监测突触小泡实时回收培养的海马神经元。

Calcium Mobilisation Assay in Response to Chemokine Stimulation
Author:
Date:
2012-05-05
[Abstract]  This assay is used to measure calcium mobilization in lymphocytes (either in primary cells or cell lines) in response to chemokine stimulation using ratiometric analysis. It has also been used for measuring TCR mediated calcium flux. In addition, the same labeling procedure with the addition of brilliant black (a quenching agent) (Sigma-Aldrich, catalog number: 211842) in the loading buffer (at 100 μM) allows for quantification using the FLIPR system on poly-D-lysine plates. Probenicid is an anion-exchange protein inhibitor and prevents the extrusion of the dyes by organic ion transporters. [摘要]  这篇文章所述分析是应用参比分析来测量趋化因子刺激下淋巴细胞(原代细胞或细胞株)中钙离子含量的变化。它也被用于测量T细胞受体介导的钙通量。另外,带有外加溶于上样缓冲液(浓度100 μM)的亮黑(一种淬灭剂,Sigma公司,编号 # 211842)的相同标签程序允许在多聚D-赖氨酸板上定量使用FLIPR 系统。羧苯磺胺是一种阴离子交换蛋白抑制剂,能防止有机离子转运蛋白挤压燃料。

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