| Product Analysis of Starch Active Enzymes by TLC
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Author:
Date:
2015-10-20
[Abstract] Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the reaction product(s) of starch active enzymes, which can contain α-1, 4 linked and α-1, 6 linked glucose. This was first published in Molecular Microbiology (Cockburn et al., 2015). The TLC protocol is an adapted version of that published by Robyt and Mukerjea (Robyt and Mukerjea, 1994). For a summary of the products generated by starch ...
[摘要] 薄层色谱（TLC）是用于检测单糖通过寡糖的存在的有用技术，尽管它需要针对所分析的特定糖进行优化。 在这里我们提出一种可视化的淀粉活性酶，可以包含α-1,4连接和α-1,6连接葡萄糖的反应产物的方法。 这首次发表于Molecular Microbiology（Cockburn等人，2015年）。 TLC方案是由Robyt和Mukerjea（Robyt和Mukerjea，1994）发表的适应版本。 对于由淀粉活性酶产生的产物的概述，参见Hii等人（2012）的综述。
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| Purification of Starch Granules from Arabidopsis Leaves and Determination of Granule-Bound Starch Synthase Activity
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Author:
Date:
2014-12-05
[Abstract] Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis ...
[摘要] 淀粉构成植物中最重要的碳储量，由支链支链淀粉和线性直链淀粉组成。 后者仅由颗粒结合的淀粉合酶（GBSS，EC 2.4.1.21）合成。 在这里我们报告一个容易重现，具体和高度敏感的协议，其中包括完整的淀粉颗粒从拟南芥叶中分离和随后的GBSS活动的确定。 我们已经应用这种方法来研究营养生长的日变化周期中和在拟南芥中的光周期过渡到开花期间的GBSS活性（Tenorio等人，2003; Ortiz-Marchena& em> et al。，2014）。
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