Roswell Park Memorial Institute (RPMI) 1640 medium

罗斯威尔公园纪念研究所（RPMI）1640培养基

公司名称: Thermo Fisher Scientific

产品编号: 31870-082

Thrombopoietin-independent Megakaryocyte Differentiation of Hematopoietic Progenitor Cells from Patients with Myeloproliferative Neoplasms

Chloe A. L. Thompson-Peach, Johannes Foßelteder, Andreas Reinisch and Daniel Thomas

Published online: 2023-01-20

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PBMC-MSC Co-cultures for Induction of Treg Generation

Author:

Sara M. Melief, C. L. M. Schrama and Helene Roelofs,

Date:

2015-01-20

[Abstract] To assess the capacity of multipotent stromal cells (MSC) to induce the generation of Tregs, transwell co-cultures were performed as well as cultures with MSC-conditioned medium (CM). In short, peripheral blood mononuclear cells (PBMC) were co-cultured with allogeneic MSC or CM for one week followed by one week of culture in the absence of MSC. [摘要] 为了评估多潜能基质细胞（MSC）诱导Treg产生的能力，进行Transwell共培养以及具有MSC条件培养基（CM）的培养。简而言之，将外周血单核细胞（PBMC）与同种异体MSC或CM共培养一周，然后在不存在MSC的情况下培养一周。

Monocyte-MSC Co-cultures

Author:

Sara M. Melief, C. L. M. Schrama and Helene Roelofs,

Date:

2015-01-20

[Abstract] To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage colony-stimulating factor (M-CSF). Monocytes were isolated from peripheral blood mononuclear cell (PBMC) populations by magnetic activated cell sorting (MACS) using CD14 microbeads. [摘要] 为了评估多潜能基质细胞（MSC）对单核细胞的作用，在MSC条件培养基（CM）中对新鲜分离的单核细胞进行3天培养。作为对照条件，用低剂量巨噬细胞集落刺激因子（M-CSF）刺激单核细胞。通过磁激活细胞分选（MACS）使用CD14微珠从外周血单核细胞（PBMC）群体中分离单核细胞。

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Roswell Park Memorial Institute (RPMI) 1640 medium

罗斯威尔公园纪念研究所（RPMI）1640培养基

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