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Lysine

L-赖氨酸
公司名称: Sigma-Aldrich
产品编号: L5501
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Imaging Thick Lymph Node Tissue Sections
Author:
Date:
2016-09-20
[Abstract]  Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled antibodies. The thick tissue sections (150-200 μm depth) allow for the detection of cell clustering that is typically under-represented in thin sections (10-20 μm) used for conventional confocal microscopy. Application of 2-photon microscopy facilitates imaging through the thick volume of the vibratome sections. In combination with automated image ... [摘要]  我们的协议描述了一个简单的程序，通过双光子显微镜成像厚淋巴结切片。 使用vibratome（振动切片机）切割淋巴结以产生组织切片，然后可用荧光标记的抗体染色。 厚组织切片（150-200μm深度）允许检测细胞聚集，其通常在用于常规共聚焦显微镜的薄切片（10-20μm）中表现不足。 双光子显微镜的应用有利于成像通过厚的体积的vibratome部分。 与自动图像处理软件组合，厚的淋巴结横截面图像还有助于在组织的相对大的区域内的细胞事件的定量，从而提供关于感兴趣的细胞事件的空间分布的更清晰的图像（例如 。，T细胞聚类）。 该方法也可以容易地应用于其它组织，例如脾或皮肤。

Phenol-based Total Protein Extraction from Lily Plant Tissues
Author:
Date:
2015-02-20
[Abstract]  The phenol-based total protein extraction method is unique in that water-soluble components such as polyphenolic compounds and nucleic acids can be easily removed. Thus, total protein is free from contaminants and allows for high quality two–dimensional gel electrophoresis. The phenol-based extraction of total protein was used in various lily organs and may likely apply to other plants whose content of polyphenolics is high (Note 1). An additional advantage of this extraction method is that nucleic acids can be easily removed and thus, avoid adverse effects of nucleic acids on protein ... [摘要]  酚类总蛋白提取方法的独特之处在于可以容易地除去多酚化合物和核酸等水溶性成分。 因此，总蛋白不含污染物，并允许高质量的二维凝胶电泳。 总蛋白的苯酚提取用于各种百合器官，可能适用于多酚含量高的其他植物（注1）。 该提取方法的另一个优点是，可以容易地除去核酸，从而避免核酸对凝胶中蛋白质分辨的不利影响。 这种方法从Hurkman和Tanaka（1986）的修改。

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