| Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
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Author:
Date:
2018-08-20
[Abstract] Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by applying “split-marker” deletion strategy (Catlett et al., 2003) with PEG-mediated protoplast transformation (Yuan et al., 2008; Martín, 2015).
[摘要] 禾谷镰刀菌是一种破坏性的植物病原体，具有令人印象深刻的代谢多样性。 基因缺失是基因功能研究的重要且有用的方法。 在这里，我们提出了一个协议，通过应用“分裂标记”删除策略（Catlett et al。，2003）与PEG介导的原生质体转化（Yuan 等。，2008;Martín，2015）。
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| Measurement of Cellular Redox in Pollen with Redox-Sensitive GFP (roGFP) Using Live Cell Imaging
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Author:
Date:
2015-03-05
[Abstract] Redox homeostasis is a fundamental property of living cells and responds actively to both cellular metabolism and external stimulus. The development of redox-sensitive GFP (roGFP) enables dynamic monitoring of changes in cellular redox poise (Hanson et al., 2014). When excited alternatively at 405 nm and 488 nm, these probes exhibit significant opposing shifts at the emission spectra (505-530 nm), which enables ratiometric measurement of relative redox values. A more oxidized environment results in a higher 405/488 ratio. Previously, successful application of roGFPs in leaf epidermis ...
[摘要] 氧化还原稳态是活细胞的基本特性，并对细胞代谢和外部刺激有积极的反应。 氧化还原敏感性GFP（roGFP）的发展使得能够动态监测细胞氧化还原时间的变化（Hanson等人，2014）。 当在405nm和488nm交替激发时，这些探针在发射光谱（505-530nm）处显示出显着的相反偏移，这使得能够比较测量相对氧化还原值。 更加氧化的环境导致更高的405/488比率。 以前，已经报道了在叶表皮或根细胞中成功应用roGFP。 在这里我们提供协议描述roGFP1成像在生长花粉管的共聚焦激光扫描显微镜的应用。
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