| Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
|
|
Author:
Date:
2020-07-05
[Abstract] Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called short-chain fatty acids. SCFAs are closely related to various aspects of the human body, so more and more researchers concentrate on SCFAs. This protocol describes, a direct injection gas chromatography detection method with a pretreatment method for extracting SCFA from mice feces by combining acidification. The corresponding sample limit of ...
[摘要] [摘要] 短链脂肪酸（SCFAs）主要由细菌在结肠中发酵未消化的碳水化合物而形成，它们的基础是碳链中碳原子的数量。碳原子少于6个的有机脂肪酸称为短链脂肪酸。SCFAs与人体的各个方面密切相关，因此SCFAs的研究越来越多。本方案描述了一种直接进样气相色谱检测法和预处理法相结合从小鼠粪便中提取SCFA的方法。相应的定量限（LOQ）和检测限（LOD）分别为0.8-1.0 mg/L和0.5-0.8 mg/L。校准曲线的相关系数大于0.999。加标回收率为80%～102%。该方法可用于小鼠粪便中SCFAs的分析测定。因此，这是一种经济、有效、重复性好的小鼠SCFAs测定方法。
[背景]短链脂肪酸（SCFA）是调节和控制生物功能的重要分子（Siri Tarino et al.，2015）。短链脂肪酸通常指含有少于六个碳原子的挥发性有机脂肪酸（Wong等人，2006年；Marette和Jobin，2015年）。主要包括异丁酸、异丁酸、异丁酸。SCFAs主要由肠道微生物群产生，在结肠中发酵未消化的碳水化合物和膳食纤维。（Henningsson等人，2001年）。许多研究表明，支链脂肪酸来自支链氨基酸和亮氨酸（Koh等人，2016）。少量支链脂肪酸是通过发酵未消化的蛋白质产生的（Cummings and ...
|
|
|
| Extraction and Quantification of Tryptophan and Kynurenine from Cultured Cells and Media Using a High Performance Liquid Chromatography (HPLC) System Equipped with an Ultra-Sensitive Diode Array Detector
|
|
Author:
Date:
2016-04-05
[Abstract] Evidence of the involvement of tryptophan and its metabolite, kynurenine, in various biological processes including cancer is constantly expanding. Analysis of cell extracts and culture media can allow for quick snapshots of the metabolic fluctuations occurring in vitro. Here, we describe a method for metabolite extraction from mammalian cells and analysis of extracted metabolites and cell culture media by HPLC with detection using an ultra-sensitive diode array detector.
[摘要] 色氨酸及其代谢物，犬尿氨酸参与包括癌症在内的各种生物过程的证据不断扩大。 细胞提取物和培养基的分析可以允许在体外发生的代谢波动的快速快照。 在这里，我们描述了一种从哺乳动物细胞代谢物提取的方法，提取代谢物和细胞培养基通过HPLC检测使用超敏二极管阵列检测器分析。
|
|
|
| A Gas Chromatography-Mass Spectrometry-Based Two Stage Assay for Measurement of in vitro myo-Inositol 3-phosphate Synthase (INO1) Activity
|
|
Author:
Date:
2015-03-05
[Abstract] This method describes an in vitro assay for measuring INO1 enzyme activity (the conversion of glucose 6-phosphate to myo-inositol 3-phosphate) in cell-free extracts. The method was first described for Plasmodium falciparum cells in MacRae et al. (2014) and consists of two parts: Part 1 describes the assay itself while part 2 describes analysis of the myo-inositol 3-phosphate product using gas chromatography-mass spectrometry (GC-MS).
[摘要] 该方法描述了用于测量无细胞提取物中INO1酶活性（葡萄糖-6-磷酸转化为肌醇-3-磷酸）的体外测定。 该方法首先在MacRae等人（2014）中描述了恶性疟原虫细胞，并由两部分组成：第1部分描述测定本身，而第2部分描述分析 使用气相色谱 - 质谱（GC-MS）测定肌醇3-磷酸产物。
|
|
|