| Phenotypic Profiling of Candida glabrata in Liquid Media
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Author:
Date:
2017-04-05
[Abstract] Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of comparing growth by eye-sight. Furthermore, the liquid method requires lower amounts of antifungals and offers a higher sensitivity. While this method has been optimized for C. glabrata it might be used for other Candida species and yeast-like fungi as well.
[摘要] 在这里，我们描述了一种用于大规模液体筛选方法的方法。 glabrata 。 这种液体介质方法比固体介质方法提供了几个明显的优点。 这包括平板阅读器的增长测量，而不是通过视力来比较增长。 此外，液体方法需要较少量的抗真菌剂并提供更高的灵敏度。 虽然这种方法已经针对C进行了优化。 glabrata 也可以用于其他念珠菌物种和酵母样真菌。
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| Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
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Author:
Date:
2016-04-05
[Abstract] We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015).
[摘要] 我们在这里提供了详细的协议，用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节（DRG）中。 解离的小鼠DRG可以通过酶消化解离成单个细胞，以获得小鼠感觉神经元的原代培养物，如Khaminets等人（2015）报道的研究中所进行的。
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| Genetic Transformation of Candida glabrata by Heat Shock
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Author:
Date:
2015-07-20
[Abstract] Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection in the human fungal pathogen Candida glabrata ATCC2001 and related strains. Furthermore, the protocol can be used to generate gene deletions in clinical isolates of Candida glabrata (C. glabrata).
[摘要] 在这里，我们报告使用热休克方法转化假丝酵母的方法。 该方案可用于单孔或96孔规模的转化。 它已被用作电穿孔方案的替代方法，以在人真菌病原体光滑假丝酵母ATCC2001和相关菌株中构建基因组规模的基因缺失集合。 此外，该方案可以用于在光滑念珠菌（Emicum Candida glabrata）（ C.glabrata ）的临床分离株中产生基因缺失。
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