Sodium phenoxide trihydrate

苯酚钠三水合物

公司名称: Sigma-Aldrich

产品编号: 318191

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Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana

Takushi Hachiya and Yuki Okamoto

Published online: 2017-05-20

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High-throughput Quantification of Ammonium Content in Arabidopsis

Author:

Izargi Vega-Mas, Asier Sarasketa and Daniel Marino,

Date:

2015-08-20

[Abstract] This protocol is a simple colorimetric assay for internal ammonium quantification in aqueous extracts from plant tissues. The method is based on the phenol hypochlorite assay (Berthelot reaction):

NH₄⁺ + hypochlorite + OH^- + phenol → indophenol

The oxidation of indophenol caused by phenol oxidation is a blue dye that is quantified at 635 nm in a spectrophotometer. Per ammonium molecule one molecule of indophenol is formed. The protocol described here is for Arabidopsis thaliana (A. thaliana) leaves and roots, although ... [摘要] 该方案是一种简单的比色测定法，用于在植物组织的水性提取物中进行内部铵定量。该方法基于苯酚次氯酸盐测定（Berthelot反应）：
NH 4+ sup + +次氯酸盐+ OH ^- +苯酚→靛酚

由酚氧化引起的靛酚的氧化是在分光光度计中在635nm处定量的蓝色染料。每个铵分子形成一个靛酚分子。这里描述的方案是用于拟南芥（拟南芥）叶和根，虽然它也适用于其他植物物种。

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Sodium phenoxide trihydrate

苯酚钠三水合物

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