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Sodium Cacodylate Buffer

二甲胂酸钠缓冲液

公司名称: Electron Microscopy Sciences
产品编号: 11654
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Morphological Quantification of Nuclei and Mitochondria in Serial Block-face Scanning Electron Microscopy Images
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2015-09-20
[Abstract]  Serial Block-face Scanning Electron Microscopy (SBF-SEM or 3D-EM) is a powerful tool to study biological structure in ultrastructural level. Quantification of cellular ultrastructure is useful to providing biological information. This technique requires not only high quality of tissue fixation and ideal sample embedding to preserve structures, but also delicate 3D image scanning and post-processing of images. We have adapted previous method to optimize the EM technique to detect and study cellular ultrastructure. Here we present the method to embed samples for 3D-EM technique and to ... [摘要]  串行块面扫描电子显微镜(SBF-SEM或3D-EM)是研究超微结构水平生物结构的强大工具。 细胞超微结构的定量对于提供生物信息是有用的。 这种技术不仅需要高质量的组织固定和理想的样品嵌入以保存结构,而且还需要精细的3D图像扫描和图像的后处理。 我们改编以前的方法优化EM技术检测和研究细胞超微结构。 在这里我们提出嵌入样品的3D-EM技术和量化的核和线粒体的形态参数的方法。

第I部分:3D-EM图像的组织嵌入

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