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Fura RedTM

Fura Red TM

公司名称: Thermo Fisher Scientific
产品编号: F3021
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Calcium Mobilisation Assay in Response to Chemokine Stimulation
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2012-05-05
[Abstract]  This assay is used to measure calcium mobilization in lymphocytes (either in primary cells or cell lines) in response to chemokine stimulation using ratiometric analysis. It has also been used for measuring TCR mediated calcium flux. In addition, the same labeling procedure with the addition of brilliant black (a quenching agent) (Sigma-Aldrich, catalog number: 211842) in the loading buffer (at 100 μM) allows for quantification using the FLIPR system on poly-D-lysine plates. Probenicid is an anion-exchange protein inhibitor and prevents the extrusion of the dyes by organic ion transporters. [摘要]  这篇文章所述分析是应用参比分析来测量趋化因子刺激下淋巴细胞(原代细胞或细胞株)中钙离子含量的变化。它也被用于测量T细胞受体介导的钙通量。另外,带有外加溶于上样缓冲液(浓度100 μM)的亮黑(一种淬灭剂,Sigma公司,编号 # 211842)的相同标签程序允许在多聚D-赖氨酸板上定量使用FLIPR 系统。羧苯磺胺是一种阴离子交换蛋白抑制剂,能防止有机离子转运蛋白挤压燃料。

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