PBS, pH 7.4

（磷酸盐缓冲盐水）溶液，pH 7.4

公司名称: Thermo Fisher Scientific

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Imaging Thick Lymph Node Tissue Sections

Author:

Jyh Liang Hor and Scott N. Mueller,

Date:

2016-09-20

[Abstract] Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled antibodies. The thick tissue sections (150-200 μm depth) allow for the detection of cell clustering that is typically under-represented in thin sections (10-20 μm) used for conventional confocal microscopy. Application of 2-photon microscopy facilitates imaging through the thick volume of the vibratome sections. In combination with automated image ... [摘要] 我们的协议描述了一个简单的程序，通过双光子显微镜成像厚淋巴结切片。使用vibratome（振动切片机）切割淋巴结以产生组织切片，然后可用荧光标记的抗体染色。厚组织切片（150-200μm深度）允许检测细胞聚集，其通常在用于常规共聚焦显微镜的薄切片（10-20μm）中表现不足。双光子显微镜的应用有利于成像通过厚的体积的vibratome部分。与自动图像处理软件组合，厚的淋巴结横截面图像还有助于在组织的相对大的区域内的细胞事件的定量，从而提供关于感兴趣的细胞事件的空间分布的更清晰的图像（例如。，T细胞聚类）。该方法也可以容易地应用于其它组织，例如脾或皮肤。

Kinetic Analysis of Monoclonal Antibody Binding to HIV-1 gp120-derived Hyperglycosylated Cores

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Jidnyasa Ingale and Richard T Wyatt,

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2015-10-05

[Abstract] Kinetic analysis of antibodies is one of the important studies for characterization of antibodies and screening of ligands. In our recent study (Ingale et al., 2014), we compared the antigenic profiles and binding characteristics of four HIV-1 envelope glycoprotein (Env) core immunogens using multiple monoclonal antibodies by Bio-Layer Light Interferometry (BLI). This technology enables real-time analysis of interactions on the surface of a fiber optic biosensor by accurately measuring kinetic constants such as K_a, K_d, and K_D in a 96-well format. [摘要] Kinetic analysis of antibodies is one of the important study for characterization of antibodies and screening of ligands. In our recent study¹, we compared the antigenic profiles and binding characteristics of four HIV-1 envelope glycoprotein (Env) core immunogens using multiple monoclonal antibodies by Bio-Layer Light Interferometry (BLI). This technology enables real-time analysis of interactions on the surface of a fiber optic biosensor by accurately measuring kinetic constants such as K_a, K_d, and K_D in a 96-well format.

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PBS, pH 7.4

（磷酸盐缓冲盐水）溶液，pH 7.4

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