| Estimation of Wound Tissue Neutrophil and Macrophage Accumulation by Measuring Myeloperoxidase (MPO) and N-Acetyl-β-D-glucosaminidase (NAG) Activities
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Author:
Date:
2015-11-20
[Abstract] The inflammatory response is essential to the reestablishment of cutaneous homeostasis following injury. In this context, leukocytes arrive at the wound site and orchestrate essential events in the wound healing process. Therefore, the quantification of specific subsets of inflammatory cells in the wound tissue is of considerable interest. The current protocol focus on a quantitative index of neutrophils and macrophages accumulation within skin lesions by measuring the specific activity of the marker enzymes Myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAG), respectively. MPO is ...
[摘要] 炎症反应对于损伤后皮肤稳态的再建立是必需的。 在这种情况下，白细胞到达伤口部位并协调伤口愈合过程中的重要事件。 因此，对伤口组织中炎性细胞的特定亚群的定量是相当感兴趣的。 通过测量标记酶髓过氧化物酶（MPO）和N-乙酰基-β-D-氨基葡萄糖苷酶（NAG）的比活性，目前的方案集中于皮肤损伤中嗜中性粒细胞和巨噬细胞积累的定量指数。 MPO以高水平存在于嗜中性粒细胞和NAG的嗜苯胺粒细胞中，在激活的巨噬细胞的溶酶体中。 这些方法允许间接估计积累到皮肤中的嗜中性粒细胞和巨噬细胞的丰度。
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| Sandwich Enzyme-linked Immunosorbent Assay (ELISA) Analysis of Plant Cell Wall Glycan Connections
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Author:
Date:
2014-04-20
[Abstract] Sandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex. In the case of plant cell wall glycans, it can reveal the existence of inter-polymers linkages, leading to better understanding of overall cell wall architectures. This development of a conventional sandwich ELISA protocol uses a carbohydrate-binding module (CBM), a small protein domain found in some carbohydrate catalysing or activating enzymes, and rat monoclonal antibodies (mAbs) which can be combined in the same ELISA plate without risk of ...
[摘要] 夹心ELISA是高度灵敏的方法，其可以用于确定两个表位是否是相同大分子或超分子复合物的一部分。 在植物细胞壁聚糖的情况下，它可以揭示存在的聚合物之间的联系，导致更好地了解整体细胞壁结构。 常规夹心ELISA方案的这种发展使用碳水化合物结合模块（CBM），在一些碳水化合物催化或活化酶中发现的小蛋白结构域和大鼠单克隆抗体（mAb），其可以组合在相同的ELISA板中，没有风险 交叉反应; 次级抗大鼠HRP抗体仅能够结合大鼠mAb，而不能结合CBM。 该协议在利兹大学的J. Paul Knox实验室开发和修改。
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