| Striatal Synaptosomes Preparation from Mouse Brain
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Author:
Date:
2018-09-05
[Abstract] The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a method to collect synaptosomes from mouse brain dorsal stratum. The synaptosomes can be used to study dopamine uptake activity, dopaminergic terminal endocytosis/trafficking using biochemical and microscopy methods, and protein analysis (Sorkina et al., 2018).
[摘要] 纹状体位于前脑的皮质下区域，它包含中等刺状神经元，胆碱能中间神经元和GABA能中间神经元，并在黑质纹状体通路中接受多巴胺能投射。 该方案提供了从小鼠脑背侧层收集突触体的方法。 突触体可用于研究多巴胺摄取活性，使用生物化学和显微镜方法的多巴胺能末端内吞作用/运输，以及蛋白质分析（Sorkina 等，，2018）。
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| Estimation of Wound Tissue Neutrophil and Macrophage Accumulation by Measuring Myeloperoxidase (MPO) and N-Acetyl-β-D-glucosaminidase (NAG) Activities
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Author:
Date:
2015-11-20
[Abstract] The inflammatory response is essential to the reestablishment of cutaneous homeostasis following injury. In this context, leukocytes arrive at the wound site and orchestrate essential events in the wound healing process. Therefore, the quantification of specific subsets of inflammatory cells in the wound tissue is of considerable interest. The current protocol focus on a quantitative index of neutrophils and macrophages accumulation within skin lesions by measuring the specific activity of the marker enzymes Myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAG), respectively. MPO is ...
[摘要] 炎症反应对于损伤后皮肤稳态的再建立是必需的。 在这种情况下，白细胞到达伤口部位并协调伤口愈合过程中的重要事件。 因此，对伤口组织中炎性细胞的特定亚群的定量是相当感兴趣的。 通过测量标记酶髓过氧化物酶（MPO）和N-乙酰基-β-D-氨基葡萄糖苷酶（NAG）的比活性，目前的方案集中于皮肤损伤中嗜中性粒细胞和巨噬细胞积累的定量指数。 MPO以高水平存在于嗜中性粒细胞和NAG的嗜苯胺粒细胞中，在激活的巨噬细胞的溶酶体中。 这些方法允许间接估计积累到皮肤中的嗜中性粒细胞和巨噬细胞的丰度。
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