Poly(vinyl alcohol)

聚乙烯醇）

公司名称: Sigma-Aldrich

产品编号: 341584

Similar Protocol

Visualizing the Cisternal Organization of Golgi Ministacks in HeLa Cells by Side-averaging

Divyanshu Mahajan, Hieng Chiong Tie and Lei Lu

Published online: 2023-04-20

Bio-protocol()

Company-protocol()

Other protocol()

Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs)

Author:

Mickaël M. Ménager and Dan R. Littman,

Date:

2016-11-20

[Abstract] The aim of this protocol is to describe how to measure and quantify the amount of HIV-1 particles and dextran molecules internalized in human monocyte derived dendritic cells (MDDCs), using three different techniques: flow cytometry, quantitative PCR and confocal microscopy. [摘要] 本协议的目的是描述如何使用三种不同的技术：流式细胞术，定量PCR和共聚焦显微镜来测量和量化人类单核细胞衍生树突细胞（MDDCs）中内在的HIV-1颗粒和葡聚糖分子的量。

[背景] 开发此协议是为了评估在人单核细胞衍生的树突细胞中肌动蛋白成核破坏时HIV-1内化的变化。在shRNA筛选后，识别对于HIV-1从树突状细胞转移到T细胞重要的基因，我们观察到肌动蛋白成核的中断导致从富含肌动蛋白的树突到水泡的转变，由于过量的肌动球蛋白收缩。结果，观察到HIV-1转移的减少和由于水泡收缩驱动的巨噬细胞增多引起的HIV-1内化的增加。我们的结论是，肌动蛋白成核和稳定的效应器是维持艾滋病毒1对肌动蛋白丰富的树突和限制其内吞作用，有效转移到T淋巴细胞的关键（Menager和Littman，2016）。

Single Molecule RNA FISH in the Mammalian Oocyte

Author:

Denisa Jansova,

Date:

2015-12-05

[Abstract] RNA fluorescence in situ hybridization is a method to localize and measure gene expression in individual cell or tissue. Using multiple specific fluorescently labeled oligonucleotides greatly increases signal-to-noise ratio and thus enables detection of single RNA molecule. Around forty different DNA oligonucleotides designed to common RNA target and labeled with single fluorophore at 3´ terminus hybridizes with target RNA in fixed cells. We adapt this method to visualize target RNA in the mammalian oocyte. The ability to detect single transcript in the mammalian oocyte was ... [摘要] RNA荧光原位杂交是定位和测量个体细胞或组织中的基因表达的方法。使用多个特异性荧光标记的寡核苷酸大大增加信噪比，从而使得能够检测单个RNA分子。大约四十个不同的DNA寡核苷酸设计为常见的RNA靶标，并在3'端用单个荧光团标记，与固定细胞中的靶RNA杂交。我们适应这种方法可视化目标RNA在哺乳动物卵母细胞。在哺乳动物卵母细胞中检测单个转录物的能力由于其大的细胞大小而具有挑战性。该方法由四个简单的步骤组成：固定，预稳定化，杂交和成像。该方案适应这种大的非附着细胞以显现母体RNA。
各种荧光团的组合允许检测更多的RNA靶标。该方法可以与细胞器标记一起使用或用免疫荧光方案扩增。

产品评论

Poly(vinyl alcohol)

聚乙烯醇）

免责声明