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Eppendorf Safe-Lock Tubes, 1.5 mL, PCR clean, colorless

Eppendorf安全锁管

公司名称: Eppendorf
产品编号: 0030123328
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Plate Assay to Determine Caenorhabditis elegans Response to Water Soluble and Volatile Chemicals
Author:
Date:
2018-02-20
[Abstract]  The nematode Caenorhabditis elegans is widely used for behavioral studies ranging from simple chemosensation to associative learning and memory. It is vital for such studies to determine optimal concentrations of attractive and aversive chemicals that C. elegans can sense. Here we describe a resource localization assay in which a chemical compound of interest is placed in two compartments of a quadrant plate in order to determine optimal concentrations of the chemical in behavioral studies. Using the assay, we determined the optimal concentration of a water-soluble ... [摘要]  线虫<秀秀隐杆线虫广泛用于从简单的化学感应到联想学习和记忆的行为研究。 这些研究对于确定吸引和厌恶化学物质的最佳浓度至关重要。="" 线虫可以感觉到。="" 在这里我们描述了一种资源定位测定法,其中将感兴趣的化学化合物置于象限板的两个隔室中以确定化学品在行为研究中的最佳浓度。="" 使用该测定,我们确定了水溶性引诱剂kcl的最佳浓度,作为研究关联学习和记忆的无条件刺激。="">

【背景】线虫线虫广泛用作动物行为研究的模式生物。 ℃。秀丽隐杆线虫感知主要由最大的化学感受器官(Ward,1973; Dusenbery,1974; Bargmann和Horvitz,1991; Bargmann等人)等介导的各种水溶性和挥发性化学物质。 1993)。行为研究必须确定可以被感知的化学物质的精确浓度。线虫。为了确定用于C的水溶性引诱剂的最佳浓度。线虫,Wicks等人(2000)使用象限琼脂平板进行行为测定,其中感兴趣的化学物质与琼脂在两个隔室中混合,并且该测定已广泛用于许多化学品(例如Jansen等人,2002; Ortiz等人,2009; Murayama和Maruyama,2013; Sassa&lt; em&gt; et al。,2013)。趋化性分析也被用于测量C的敏感性。 ...

Preparation of Single Cell Suspensions from Mouse Aorta
Author:
Date:
2016-06-05
[Abstract]  Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery tertiary lymphoid organs in the adventitia (Grabner et al., 2009; Hu et al., 2015). Various and diverse types of immune cells have been identified in the aorta adventitia vs atherosclerotic plaques (Elewa et al., 2016; Galkina et al., 2006; Lotzer et al., 2010; ... [摘要]  动脉粥样硬化是动脉壁的慢性炎性疾病,其特征在于脂质沉积,斑块形成和免疫细胞浸润。先天性和适应性免疫细胞在疾病发展期间浸润动脉。此外,晚期疾病导致外膜中动脉三级淋巴器官的形成(Grabner等人,2009; Hu等人,2015)。已经在主动脉外膜vs动脉粥样硬化斑块中鉴定了各种不同类型的免疫细胞(Elewa等人,2016; Galkina等人,2006; Lotzer等人, 2010; Mohanta等人,2016; Mohanta等人,2014; Moos等人,2010; Mohanta等人,2010; 2005; Srikakulapu et al。,2016; Zhao et al。,2004)。根据动物的年龄,用于获得单细胞悬浮液的方案和小鼠的饮食条件,存在关于主动脉中免疫细胞的数量和亚型的矛盾报告(Campbell等, 2012; Clement等人,2015; Galkina等人,2006; Kyaw等人,2012)。使用不同的方案,主动脉中免疫细胞的数目差异多达十倍(Butcher等,2012; Galkina等,2006; Gjurich等,2015; Grabner等人,2009; ...

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