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Author:
Date:
2016-07-05
[Abstract] Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut it into 1 mm long rings. Next, the rings were cultured in growth factor-reduced matrigel polymerized at 37 °C. Angiogenesis was assessed at 7 days by using an inverted microscope platform.
[摘要] 血管生成是来自预先存在的血管芽的血管生长的性质和病理过程。 它在癌症和心血管疾病中起重要作用。 主动脉环测定是血管生成的一种方法。 在这个实验中，我们使用大鼠主动脉作为材料，清洁主动脉的周围组织，并将其切成1 mm长的环。 然后将环在37℃下聚合的生长因子减少的基质胶中培养。 通过使用倒置显微镜平台在7天评估血管发生。
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