| Phenotypic Profiling of Candida glabrata in Liquid Media
|
|
Author:
Date:
2017-04-05
[Abstract] Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of comparing growth by eye-sight. Furthermore, the liquid method requires lower amounts of antifungals and offers a higher sensitivity. While this method has been optimized for C. glabrata it might be used for other Candida species and yeast-like fungi as well.
[摘要] 在这里，我们描述了一种用于大规模液体筛选方法的方法。 glabrata 。 这种液体介质方法比固体介质方法提供了几个明显的优点。 这包括平板阅读器的增长测量，而不是通过视力来比较增长。 此外，液体方法需要较少量的抗真菌剂并提供更高的灵敏度。 虽然这种方法已经针对C进行了优化。 glabrata 也可以用于其他念珠菌物种和酵母样真菌。
|
|
|
| A Bioassay Protocol for Quorum Sensing Studies Using Vibrio campbellii
|
|
Author:
Date:
2016-07-20
[Abstract] Quorum Sensing (QS), or bacterial cell-to-cell communication, is a finely-tuned mechanism that regulates gene expression on a population density-dependent manner through the production, secretion and reception of extracellular signaling molecules termed autoinducers (AIs). Given that QS plays an important role in bacterial biofilm formation and virulence factor production in many pathogenic strains, QS disruptors have become a hot topic in current antimicrobial research. There are several reporter strains exhibiting QS-regulated phenotypes that have been engineered for the identification of ...
[摘要] Quorum Sensing（QS）或细菌细胞与细胞间的通信是一种微调的机制，通过称为自体诱导物（AI）的细胞外信号分子的产生，分泌和接受，以群体密度依赖性方式调节基因表达。鉴于QS在许多致病菌株中的细菌生物膜形成和毒力因子产生中起重要作用，QS干扰物已经成为当前抗微生物研究的热门话题。存在几种报道菌株，其表现出QS-调节的表型，其已被工程化用于鉴定QS抑制剂，包括例如颜料产生（González和Keshavan，2006; Steindler和Venturi，2007），gfp < （gonzález和keshavan，2006;="" steindler和venturi，2007）或致死性基因融合下游qs控制的启动子（weiland-bräuer=""> et al 。，2015）。使用三个平行的QS电路，生物发光海洋细菌（Vibrio campbellii）（以前称为harveyi，Lin等人，2010）构成复杂的革兰氏阴性模型，存在广泛的知识，包括一系列突变生物传感器。在中。 campbellii ...
|
|
|