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Sodium iodide

碘化钠

公司名称: Sigma-Aldrich
产品编号: 383112
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Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae
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2016-07-20
[Abstract]  Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond serving as Pi store or energy source to replace ATP. For instance, in yeast polyP levels have been related to stress adaptation and this molecule has been shown to be the substrate for polyphosphorylation of proteins. Here we describe two different methods to purify polyP from the yeast Saccharomyces cerevisiae and the subsequent protocol to ... [摘要]  无机多磷酸盐(polyP)是存在于原核和真核生物中的线性聚合物,由通过磷酸酐键连接的三至数百个正磷酸盐残基制成。 这种分子的生物学作用超越了作为P 1储存或能量源以代替ATP。 例如,在酵母中,polyP水平与应激适应相关,并且该分子已经显示为蛋白质多磷酸化的底物。 在这里,我们描述了从酵母酿酒酵母中纯化polyP的两种不同方法和随后的通过分光光度法测量在酶水解纯化的polyP时产生的Pi来定量polyP水平的方案。 必须注意的是,所使用的纯化方案极大地影响所获得的polyP值。

图1. polyP的酶水解

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