{{'Search' | translate}}
 

NP-40 (10% in H2O)

NP-40(10%在H 2 O中)

公司名称: MBL International
产品编号: JM-2111-100
Bio-protocol()
Company-protocol()
Other protocol()

Cell Cycle Analysis using Propidium Iodide Staining with GFP Detection
Author:
Date:
2012-04-05
[Abstract]  Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell cycle (propidium iodide, PI staining) of GFP positive cells vs GFP negative cells is needed. Usually simple fixation of cells with 70% EtOH for PI staining tends to quench GFP signal; paraformaldehyde (PFA) fixation before ETOH fixation could help to sustain the GFP signal. [摘要]  用一个GFP标记的质粒转染哺乳动物细胞过表达或者调低目的基因是一种最常用的操纵基因表达的方法。想要在细胞循环中检测目的基因的功能,分析GFP阳性细胞和GFP阴性细胞的细胞循环(碘化丙啶,PI染色)是必要的。通常为PI染色用70 %乙醇简单固定细胞淬火GFP信号,在乙醇固定前用低聚甲醛(PFA)固定能帮助染GFP信号。

产品评论