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TRIS ULTRAPURE

TRIS超声
公司名称: Thermo Fisher Scientific
产品编号: 50-213-709
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An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Author:
Date:
2012-06-20
[Abstract]  One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible. Hyperhosphorylated proteins usually migrate slowlier than dephosphorylated proteins. However, not all phosphorylated proteins can be readily detected, due to sub-optimal sample preparation and electrophoresis conditions. Here, an improved method is described that can detect phosphorylation of yeast proteins ranging from 15 kD to 200 kD. The improvement in gel electrophoresis should also be applicable to mammalian culture cells. [摘要]  一维聚丙烯酰胺凝胶电泳已被成功用于检测蛋白磷酸化。这种方法是非常简便的并且高再现性的。 高磷酸化蛋白通常比去磷酸化蛋白迁移得慢。尽管如此，并不是所有的磷酸化蛋白都很容易的检测到，由于欠佳的样品制备和电泳条件。在此，我描述了一种能够检测到大小范围是15 kd 到 200 kd的酵母磷酸化蛋白的改进方法。凝胶电泳的改进同样适用于哺乳动物培养的细胞。

In vitro Protein Kinase Assay
Author:
Date:
2012-06-05
[Abstract]  This protocol will describe experimental procedures for an in vitro kinase assay of the yeast protein kinase Sch9. This protocol can be tailored to detect kinase activity of other yeast protein kinase. [摘要]  这个实验方案将描述酵母蛋白激酶Sch9体外激酶活性检验的实验程序。这个规程可以适用于检测其它酵母蛋白激酶激酶活性。

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