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Buffer QC

缓冲液QC

公司名称: QIAGEN
产品编号: 19055
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Yeast DNA Replication 2D Gel Protocol
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Date:
2012-07-05
[Abstract]  Two-dimensional agarose gel electrophoresis (2D gel) analysis is used extensively as a method to detect origins of replication. Here, I present a simplified method for the isolation of yeast genomic DNA for 2D gel analysis from a small number of yeast cells. This DNA isolation method is simpler and less time consuming than the traditional method that involves CsCl density gradient centrifugation. This method could be modified for 2D gel analysis in other organisms as well. [摘要]  双向琼脂糖凝胶电泳是一种被广泛用来检测DNA复制起点的方法。在这里,我介绍一种从少量酵母细菌中提取其基因组DNA用于双向电泳分析的简便方法。与传统的提取DNA的方法相比较,这种涉及到氯化铯密度梯度离心的方法更加简便和省时。而且,对这种方法进行适当的改进,从而使其也可适用于其他有机物的双向电泳分析。

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