| Glioma Associated Stem Cells (GASCs) Isolation and Culture
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Author:
Date:
2015-05-05
[Abstract] Glioma Associated Stem Cells (GASCs) represent a population of non-tumorigenic multipotent stem cells hosted in the microenvironment of human gliomas. In vitro, these cells are able, through the release of exosomes, to increase the biological aggressiveness of glioma-initiating cells. The clinical importance of this finding is supported by the strong prognostic value associated with the GASCs surface immunophenotype thus suggesting that this patient-based approach can provide a groundbreaking method to predict prognosis and to exploit novel strategies that target the tumor stroma ...
[摘要] 胶质瘤相关干细胞（GASC）代表在人类神经胶质瘤的微环境中存在的非致瘤性多能干细胞群体。 在体外，这些细胞能够通过外来体的释放增加胶质瘤起始细胞的生物攻击性。 该发现的临床重要性得到与GASCs表面免疫表型相关的强预后价值的支持，因此表明这种基于患者的方法可以提供开创性的方法来预测预后并开发靶向肿瘤基质的新策略（Bourkoula等人， et al。，2014）。
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| Generation of Mouse Bone Marrow-Derived Macrophages (BM-MFs)
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Author:
Date:
2012-06-20
[Abstract] Generating mouse macrophages from bone-marrow progenitor cells is a useful tool to study biological functions of mouse macrophages. Macrophages are one of the major populations of phagocytes and play many different roles during inflammatory process initiation and termination.
[摘要] 获得骨髓祖细胞来源的小鼠巨噬细胞是一种研究小鼠巨噬细胞生物学功能的有用工具，这个 实验方法在我们《自然》杂志的论文上有记载（请看参考文献）。
通讯作者: Ivan Zanoni and Francesca Granucci.
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| Generation of Mouse Bone Marrow-Derived Dendritic Cells (BM-DCs)
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Author:
Date:
2012-06-20
[Abstract] Generating mouse dendritic cells from bone-marrow progenitor cells is a useful tool to study biological functions of mouse dendritic cells. Dendritic cells are one of the major populations of phagocytes able to activate both innate and adaptive immune cells.
[摘要] 获得骨髓祖细胞来源的的小鼠树状细胞是一种研究小鼠树状细胞生物学功能的有用工具，这个实验方法在我们《自然》杂志的论文上有记载（请看参考文献）。
通讯作者: Ivan Zanoni and Francesca Granucci.
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