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Imidazole

咪唑

公司名称: Sigma-Aldrich
产品编号: 436151
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Purification of His-ubiquitin Proteins from Mammalian Cells
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Date:
2012-09-05
[Abstract]  This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just the p53-ubiquitinate. The present protocol can be used in general for His-tag proteins expressed in mammalian cells. [摘要]  该实验方案的中文版正在准备中...

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