| Detection of CLEC5A-JEV Interaction by ELISA
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Author:
Date:
2012-10-05
[Abstract] JEV (Japanese encephalitis virus) belonging to Flaiviridae interacts with CLEC5A (C-type lectin domain family 5, member A), a member of C-type lectin associated with DAP12 signaling protein and expressed on myeloid cell, as the same extent as Dengue virus. This protocol is used to perform and determine the interaction between purified JEV particles with human and murine CLEC5A.Fc fusion proteins by ELISA method. The different strain and batch of purified JEV as well as purity of CLEC5A.Fc fusion protein might affect the result of absorbance. Modifications ...
[摘要] 该实验方案的中文版正在准备中．．．
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| ELISA Measurement of Mouse IL-2
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Author:
Date:
2012-01-05
[Abstract] Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and co-culturing conditions. Upon slightly modification and optimization, this protocol can also be adapted to quantitatively measure other secreted proteins and bio-molecules.
[摘要] 白细胞介素-2（IL-2）是由免疫系统激活所必需的T细胞分泌的细胞因子。 该方案常规用于在各种刺激和共培养条件下定量培养的淋巴细胞的上清液中的IL-2浓度。 在稍微修改和优化后，该方案也可以适用于定量测量其他分泌的蛋白质和生物分子
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