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Cdc42 Antibody (P1)

Cdc42抗体(P1)

公司名称: Santa Cruz Biotechnology
产品编号: sc-87
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RhoGTPase Activation Assay
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Date:
2012-10-05
[Abstract]  This protocol describes procedures to assay for GTP-bound (active) form of small GTPases of the Rho superfamily in human brain cancer (glioma) cell lines but can also be applied to cells or tissues of other origins. The principle of assay is based on the property of these active GTPases to interact with their specific effectors (e.g. Rhotekin for RhoA; p21-activated kinase (PAK) for Rac1 and Cdc42. In essence, Rhotekin or PAK1 are expressed in the form of GST-fusion protein to pull down the corresponding active GTPases. [摘要]  该实验方案的中文版正在准备中...

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