T75 flasks

T75烧瓶

公司名称: Corning

产品编号: 430725

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A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells

Zhe Han, Bindhu K. Madhavan, Serap Kaymak, Peter Nawroth and Varun Kumar

Published online: 2022-08-20

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Transwell Co-culture of Bone Marrow Macrophages with Tumor Cells

Author:

Mackenzie K. Herroon and Izabela Podgorski,

Date:

2014-05-05

[Abstract] Bone is a primary site of metastasis from prostate and breast cancers. Bone marrow macrophages (BMMs) are mediators of inflammatory processes and are thought to promote tumor growth in the skeletal sites. In order to elucidate how their interactions with tumor cells impact aggressiveness of metastatic tumors in bone in vitro methods are required. By employing a system in which BMMs and tumor cells are grown separately, yet share the media and exchange soluble factors, contribution of each cell type in the context of BMM-tumor cell relationship in the bone marrow can be investigated. ... [摘要] 骨是来自前列腺和乳腺癌的原发转移部位。骨髓巨噬细胞（BMM）是炎症过程的介质，并且被认为促进骨骼部位中的肿瘤生长。为了阐明它们与肿瘤细胞的相互作用如何影响骨髓中转移性肿瘤的侵袭性，需要体外方法。通过采用其中BMM和肿瘤细胞分开生长，但共享培养基和交换可溶性因子的系统，可以研究在骨髓中BMM-肿瘤细胞关系的上下文中每种细胞类型的贡献。该系统的另外的优点包括研究以下的能力：1）共培养时BMM和肿瘤细胞中的表型改变; 2）蛋白质和基因表达的细胞特异性调节;和3）对增殖和细胞存活的影响。值得注意的是，该transwell共培养系统不限于BMM和肿瘤细胞，并且可以容易地修饰以包括骨髓微环境的其他组分（例如，脂肪细胞，基质细胞，成骨细胞）。

Viral Immunofluorescence with Rift Valley Fever Virus Infected MEFs in a 96 Well Plate

Author:

Theresa S. Moser and Sara Cherry,

Date:

2012-12-05

[Abstract] Immunofluorescence is a method to detect viral infection in multiple types of host cells. This procedure can be adapted for both high-throughput and low-throughput assays for any virus for which there are antibodies available. Time of infection and virus multiplicity of infection (MOI) vary and should be optimized for each virus and host cell type. Here we give an example of viral immunofluorescence in a 96 well plate with a Rift Valley fever virus (RVFV, strain MP12) infection in mouse embryonic fibroblasts (MEF). [摘要] 免疫荧光是检测多种类型的宿主细胞中的病毒感染的方法。该方法可适用于任何具有可用抗体的病毒的高通量和低通量测定。感染时间和病毒感染复数（MOI）不同，应该对每种病毒和宿主细胞类型进行优化。在这里我们给出在小鼠胚胎成纤维细胞（MEF）中使用Rift Valley热病毒（RVFV，菌株MP12）感染的96孔板中的病毒免疫荧光的实例。

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