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公司名称: Promega

产品编号: P1171

Single Oligonucleotide Capture of RNA And Temperature Elution Series (SOCRATES) for Identification of RNA-binding Proteins

Allen T. Yu, Disha Aggarwal, Darryl Pappin and David L. Spector

Published online: 2022-12-20

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UV Cross-linking Assay and Competition Assay

Author:

Ying-Wen Huang and Yau-Heiu Hsu,

Date:

2012-12-20

[Abstract] UV cross-linking assay is a standard method used to detect protein-RNA interaction. This method takes advantage of UV irradiation to trigger the formation of the covalently bonded RNP (ribonucleoprotein) complex that is more stable and makes it possible to be isolated in the denaturing conditions. Briefly, ³²P-labeled RNA probe and proteins are incubated to form RNP complexes spontaneously; the mixture is then exposed to UV irradiation, followed by treatment with ribonuclease to remove RNA fragments not covalently bound to protein. The oligoribonucleotide-protein complexes are ... [摘要] 该实验方案的中文版正在准备中．．．

Synthesis of 5’ end-labeled RNA

Author:

Harald Putzer,

Date:

2012-07-20

[Abstract] 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P³² labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not work on 5’ PPP RNA and require a 5’ P substrate. The activity of certain endoribonucleases like Escherichia coli (E. coli) RNase E or Bacillus subtilis (B. subtilis) RNase Y can be stimulated by a 5’ P moiety.
[摘要] 该实验方案的中文版正在准备中．．．

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