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Author:
Date:
2013-02-05
[Abstract] Peripheral blood mononuclear cells (PBMCs) consist of chiefly of lymphocytes and monocytes. Purified PBMCs are used in vitro to evaluate a variety of functions of lymphocytes viz; a) proliferation to mitogenic (PHA, Con-A) stimulation, b) monitoring of prior sensitisation in antigen recall assays by scoring lymphocyte proliferation, c) immunophenotyping for surface markers as well as intracellular molecules in monocytes and lymphocytes etc. Activation of monocytes/macrophages by small molecules, cytokines and pathogen components can also be monitored. PBMCs can also be used for a ...
[摘要] 外周血单核细胞（PBMC）主要由淋巴细胞和单核细胞组成。 纯化的PBMC在体外用于评价淋巴细胞的多种功能， a）增殖至促有丝分裂（PHA，Con-A）刺激，b）通过评估淋巴细胞增殖来监测抗原召回试验中的先前致敏，c）表面标志物以及单核细胞和淋巴细胞等中的细胞内分子的免疫表型。 也可以监测小分子，细胞因子和病原体组分的巨噬细胞。 PBMC还可以用于多种结构和功能研究，用于解决人免疫学中的问题，例如细胞凋亡评分和细胞因子以及其他介体的体外生产。
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