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Recombinant Murine IL-2

重组鼠IL-2
公司名称: PeproTech
产品编号: 212-12
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Reconstitution of Lymphopaenic Mice with Regulatory and Conventional T cell Subsets
Author:
Date:
2016-05-20
[Abstract]  Transfer of mature T cells into immunodeficient mice results in sub-optimal reconstitution of the peripheral T cell pool. Under lymphopenic conditions, dendritic cells are released from tonic control by regulatory T cells (Tregs), and consequently drive activation and proliferation of low affinity T cells specific for endogenous antigens. This oligoclonal proliferation results in a T cell population dominated by T cells possessing an effector/memory phenotype and a limited TCR repertoire. Oligoclonal expansion can be prevented by selectively reconstituting the Treg compartment prior to T cell ... [摘要]  将成熟T细胞转移到免疫缺陷小鼠中导致外周T细胞库的次优重建。在淋巴细胞减少症状下，树突状细胞通过调节性T细胞（Tregs）从强直对照释放，并因此驱动对内源性抗原特异的低亲和力T细胞的激活和增殖。这种寡克隆增殖导致由具有效应/记忆表型和有限TCR库的T细胞支配的T细胞群。可以通过在T细胞转移之前选择性重建Treg区室来防止寡克隆扩增（Bolton等人，2015）。已经在免疫缺陷小鼠品系例如Rag-1 /或 Rag-2 -/- 中测试了淋巴细胞减少小鼠的Treg区室的重建。 小鼠，以及在通过致死性全身照射作为调节骨髓移植（BMT）的瞬时淋巴细胞减少的免疫小鼠中。将纯化的Treg转移到这些宿主中，结合用外源IL-2处理7天，足以重建Treg区室并减少树突细胞共刺激分子的表达，这是防止自身反应性T细胞不适当扩增的关键过程。在Treg重建后转移的T细胞不经历快速的自发增殖，而是进行慢的内稳态分裂以用天然T细胞重新增殖T细胞库，从而允许外周T细胞库的最佳重建。

Generation of Mouse iNKT Cell Lines
Author:
Date:
2013-03-20
[Abstract]  Natural killer T (NKT) cells bridge the innate and adaptive arms of the immune system, and manipulating their effector functions can have therapeutic significances in the treatment of autoimmunity, transplant biology, infectious disease and cancer. This important lymphocyte subset regulates the immune system through their potent cytokine production following the recognition of lipid antigen present in the context of the MHC class I-like CD1d molecule, in addition their ability to directly mediate cytotoxicity. Here, we describe a method of expanding mouse invariant NKT (iNKT) cell lines ... [摘要]  自然杀伤T（NKT）细胞桥接免疫系统的先天和适应性臂，并且操纵它们的效应子功能可以在治疗自身免疫，移植生物学，传染病和癌症中具有治疗意义。 这种重要的淋巴细胞亚群通过它们在识别存在于MHC I类CD1d分子的背景中的脂质抗原之后的有效细胞因子产生，以及它们直接介导细胞毒性的能力来调节免疫系统。 在这里，我们描述了扩增小鼠不变NKT（iNKT）细胞系从使用骨髓衍生的树突状细胞从胸腺，脾或肝分离的单核细胞的方法。 这些iNKT细胞系可用于研究其共信号要求，细胞因子谱和细胞毒性功能，这将大大增强我们对iNKT细胞生物学的知识。

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