| Purification of Human Monocytes and Lymphocyte Populations by Counter Current Elutriation – A Short Protocol
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Author:
Date:
2013-12-05
[Abstract] Investigations of the activation processes involved in human monocytes and monocyte-derived macrophages and dendritic cells often required large numbers of cells that have not been possibly altered or activated by adherence to surfaces, by binding of antibodies to surface antigens during positive selection, or by release of activators by platelets or other non myeloid cells during isolation or co-culture. Human peripheral blood monocytes as well as lymphocytes from the same blood donor can be isolated by counterflow elutriation using a modification of the technique of Lionetti et al. ...
[摘要] 涉及人单核细胞和单核细胞衍生的巨噬细胞和树突细胞的激活过程的研究通常需要大量的细胞，这些细胞不可能通过附着于表面而改变或活化，通过在阳性选择期间抗体与表面抗原的结合，或通过释放 的血小板或其他非骨髓细胞在分离或共培养期间的活化剂。 人类外周血单核细胞以及来自相同血液供体的淋巴细胞可以通过逆流淘析使用Lionetti等人（1980）的技术的修改来分离，如前所述（Bobak等人 。，1986）。 从吸入抗凝血剂的单位，可以获得60-120,000个单核细胞。 这些细胞没有被活化并且已经显示在多个研究中适当地能够差别激活。
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| C1q Binding to and Uptake of Apoptotic Lymphocytes by Human Monocyte-derived Macrophages
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Author:
Date:
2013-09-05
[Abstract] To characterize macrophage gene expression profiles during the uptake of autologous apoptotic cells, we developed a unique, more physiologic system using primary human monocyte derived macrophages purified via a nonactivating isolation procedure (and in the absence of contaminating platelets, which can release stimulating signals if activated) and autologous lymphocytes as a source of apoptotic cells. The use of autologous cells as the apoptotic target rather than transformed cell lines avoids antigenic stimulation from “nonself” structures at the HLA level but also from “altered self” ...
[摘要] 为了表征在自体凋亡细胞摄取期间的巨噬细胞基因表达谱，我们开发了一种独特的，更生理的系统，使用通过非活化分离程序纯化的原代人单核细胞衍生的巨噬细胞（和在没有污染的血小板，如果激活可以释放刺激信号 ）和自体淋巴细胞作为凋亡细胞的来源。 使用自体细胞作为凋亡靶而不是转化细胞系避免了来自HLA水平上的"非自身"结构的抗原刺激，而且还由于细胞系中固有的转化而导致的"改变的自身"信号。
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