Annexin V-FITC Apoptosis Kit

膜联蛋白V-FITC凋亡试剂盒

公司名称: BioVision

产品编号: K101-100

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C1q Binding to and Uptake of Apoptotic Lymphocytes by Human Monocyte-derived Macrophages

Author:

Marie E. Benoit, Elizabeth V. Clarke and Andrea J. Tenner,

Date:

2013-09-05

[Abstract] To characterize macrophage gene expression profiles during the uptake of autologous apoptotic cells, we developed a unique, more physiologic system using primary human monocyte derived macrophages purified via a nonactivating isolation procedure (and in the absence of contaminating platelets, which can release stimulating signals if activated) and autologous lymphocytes as a source of apoptotic cells. The use of autologous cells as the apoptotic target rather than transformed cell lines avoids antigenic stimulation from “nonself” structures at the HLA level but also from “altered self” ... [摘要] 为了表征在自体凋亡细胞摄取期间的巨噬细胞基因表达谱，我们开发了一种独特的，更生理的系统，使用通过非活化分离程序纯化的原代人单核细胞衍生的巨噬细胞（和在没有污染的血小板，如果激活可以释放刺激信号）和自体淋巴细胞作为凋亡细胞的来源。使用自体细胞作为凋亡靶而不是转化细胞系避免了来自HLA水平上的"非自身"结构的抗原刺激，而且还由于细胞系中固有的转化而导致的"改变的自身"信号。

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Annexin V-FITC Apoptosis Kit

膜联蛋白V-FITC凋亡试剂盒

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