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Author:
Date:
2013-11-05
[Abstract] This protocol describes two similar methods for the extraction and methylation of fatty acids from bacterial cultures. The acid derivatization protocol (Lennen et al., 2013; Bligh and Dyer, 1959) results in the extraction and methylation of all fatty acids, both free and bound, from a bacterial culture, while the base derivatization protocol (Lennen and Pfleger, 2013) captures only bound (phospholipid, acyl-thioester) species. After extraction into hexane, the lipids may be analyzed by gas chromatography.
[摘要] 该方案描述了从细菌培养物中提取和甲基化脂肪酸的两种类似方法。 酸衍生化方案（Lennen等人，2013; Bligh和Dyer，1959）导致从细菌培养物中游离和结合的所有脂肪酸的提取和甲基化，而碱衍生化 方案（Lennen和Pfleger，2013）仅捕获结合的（磷脂，酰基 - 硫酯）物质。 在萃取到己烷中之后，可以通过气相色谱分析脂质。
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