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Author:
Date:
2014-03-20
[Abstract] Astrovirus are small, nonenveloped, single-stranded RNA viruses that cause diarrhea in a wide variety of mammals and birds. Here, we describe astrovirus propagation, purification and titration. The Caco-2 human intestinal adenocarcinoma cell line is most widely used for studying astrovirus, although other cell lines, such as 293, T84 and LLC-MK2 can be used for propagation. However, Caco2 cells are desirable for their ability to form a differentiated intestinal epithelium, mimicking the human intestine and providing a realistic model for astrovirus growth and propagation.
[摘要] 星状病毒是小的，未发育的单链RNA病毒，其在多种哺乳动物和鸟类中引起腹泻。 在这里，我们描述星状病毒繁殖，纯化和滴定。 Caco-2人肠腺癌细胞系最广泛地用于研究星形病毒，尽管其它细胞系，例如293，T84和LLC-MK2可用于繁殖。 然而，Caco2细胞是其形成分化的肠上皮的能力，模拟人的肠并且为星状病毒生长和繁殖提供现实模型所需的。
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