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Carestream® Kodak® BioMax® XAR Film

Carestream ®柯达® BioMax ® XAR Film

公司名称: Sigma-Aldrich
产品编号: F5388
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Zonal Sedimentation Analysis on Sucrose Gradients
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2014-04-20
[Abstract]  Zonal sedimentation analysis on sucrose gradients allows estimation of the molecular size of an individual protein or a protein complex by centrifugation at a constant speed under nondenaturing conditions. This method is particularly suitable for globular proteins like the influenza A virus (IAV) protein hemagglutinin (HA). Here, I describe step by step a protocol used to evaluate the oligomeric state of recombinant HA trimers (Magadan et al., 2013). [摘要]  对蔗糖梯度的区域沉降分析允许通过在非变性条件下以恒定速度离心来估计单个蛋白质或蛋白质复合物的分子大小。 该方法特别适用于如甲型流感病毒(IAV)蛋白血凝素(HA)的球状蛋白。 在这里,我逐步描述用于评估重组HA三聚体的寡聚状态的方案(Magadan等人,2013)。

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