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Author:
Date:
2016-02-20
[Abstract] Activity-dependent local mRNA translation endows synapses to remodel their structure and function (Bramham and Wells, 2007). This process is tightly controlled by the state of phosphorylation of several components of the translational machinery including initiation factors and ribosomal proteins (Buffington et al., 2014). The present protocol describes a method to prepare striatal synaptoneurosomes, from adult mice, containing both pre- and postsynaptic elements in which the level of synaptic phospho-proteins can be quantified (Biever et al., 2015).
[摘要] 活动依赖的局部mRNA翻译赋予突触重塑其结构和功能（Bramham和Wells，2007）。 该过程由包括起始因子和核糖体蛋白在内的翻译机理的几个组分的磷酸化状态来严格控制（Buffington等，2014）。 本方案描述了一种从成年小鼠制备纹状体synaptoneurosomes的方法，其含有可以量化突触磷酸蛋白水平的突触前和突触后元件（Biever等，2015）。
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Author:
Date:
2014-05-05
[Abstract] In many environments, bacteria favor a sessile, surface-attached community lifestyle. These communities, termed biofilms, are ubiquitous among many species of bacteria. In some cases, biofilms form under flow conditions. Flow chambers, and in particular microfluidic channels, can be used to observe biofilm development and physiological effects while varying nutrient conditions, flow velocities, or introducing antimicrobials to the biofilm in real time. Here, we describe a microfluidic-based kill-kinetics assay for the observation of antimicrobial effects on biofilms under flowing conditions. ...
[摘要] 在许多环境中，细菌喜欢固着的，表面附着的社区生活方式。 这些社区，称为生物膜，在许多物种的细菌中是普遍存在的。 在一些情况下，在流动条件下形成生物膜。 流动室，特别是微流体通道，可以用于观察生物膜的发展和生理效应，同时改变营养物条件，流速，或实时地将抗微生物剂引入生物膜。 在这里，我们描述了一种基于微流体的杀菌动力学测定法，观察在流动条件下对生物膜的抗微生物效应。
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