| Neurite Outgrowth Assay
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Author:
Date:
2016-01-05
[Abstract] Neurite outgrowth in culture provides an easy way to determine the effects of a particular substrate or exogenous factor on neuron behavior. Dissociated neurons can be plated on a variety of substrates and the length of the longest neurite outgrowth can be compared. Here, we describe how to isolate and dissociate dorsal root ganglion (DRG) neurons, culture them on coverslips, and measure longest neurite outgrowth.
[摘要] 文化中的神经细胞生长提供了一种简单的方法来确定特定底物或外源因子对神经元行为的影响。 分离的神经元可以铺在各种底物上，并且可以比较最长的神经突生长的长度。 在这里，我们描述如何分离和解离背根神经节（DRG）神经元，将其培养在盖玻片上，并测量最长的神经突生长。
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| Rat Aortic Ring Model to Assay Angiogenesis ex vivo
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Author:
Date:
2015-10-20
[Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of endothelial cells which are modified by repeated passages and are fully proliferative, a situation far away from physiology. In addition, not only endothelial cells are involved in this process but surrounding cells (such as pericytes, smooth muscle ...
[摘要] 血管生成是一种多因素事件，需要内皮细胞的迁移，增殖，分化和结构重排。这种血管生成过程已经使用体外试验例如Boyden腔室试验，伤口愈合试验和管形成试验进行了普遍研究。这些测定主要使用通过重复传代修饰的内皮细胞的单层，并且是完全增殖的，远离生理学的情况。此外，不仅内皮细胞参与这个过程，而且周围细胞（例如周细胞，平滑肌细胞，成纤维细胞）和支持基质也是主要的玩家。
三维主动脉环模型概括了血管生成的复杂性并结合了体外和体内模型的优点。主动脉环在化学上确定的培养环境中培养。在该系统中生长的微血管是具有周围支持细胞的管腔血管，并且与在体内血管生成期间形成的微血管基本上不可区分。可以在不存在血清分子的情况下测定促血管生成因子或抗血管生成因子的功效，否则血清分子可能干扰待测试的物质（Nicosia和Ottinetti，1990）。然而，该系统需要获得新鲜的大鼠组织，但是可以从一个主动脉制备几个样品。
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| Open-book Preparations from Chick Embryos and DiI Labeling of Commissural Axons
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Author:
Date:
2014-07-05
[Abstract] Successful neural circuit formation relies on the accurate navigation of axons towards their targets during development. Axons are guided by a combination of short-range and long-range, attractive and repulsive cues. The commissural axons of the developing spinal cord have provided an informative in vivo model for the identification of multiple axon guidance molecules and mechanisms. These axons extend ventrally from the dorsal spinal cord and cross the midline at the floor plate, before making a sharp rostral turn towards the head. This simple trajectory has facilitated the ...
[摘要] 成功的神经电路形成依赖于在发展期间轴突朝向其靶标的精确导航。轴突由短程和长程，吸引和排斥线索的组合引导。发育中的脊髓的连合轴突提供了用于多轴突导向分子和机制的鉴定的信息的体内模型。这些轴突从背侧脊髓向腹侧延伸并在底板处穿过中线，然后朝向头部进行尖锐的嘴唇转向。这个简单的轨迹有助于许多轴突指导分子的识别，因为可以容易地识别作为遗传操作的结果的定型指导决定的干扰。开放书测定是评估脊柱连合轴突的轨迹的方法。脊髓被解剖出来，在屋顶板上打开并固定平。亲脂性荧光染料DiI的点状注射用于在显微镜和分析之前追踪连合轴突轨迹。
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