| Bone Marrow-derived Endothelial Progenitor Cell Intercellular Adhesion Assay
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Author:
Date:
2016-08-20
[Abstract] Cell-cell adhesion ensures tight contacts between neighbouring cells, which is necessary for cell segregation, as well as for the morphological and functional differentiation of different tissues. Evidently there are cell-cell recognition systems that make cells of the same type preferentially adherent to one another. Homotypic cell adhesion is particularly important in mediating a range of physiological processes such as cell survival, migration and remodeling of vessels. Thus in the present study we selected two populations of endothelial progenitor cells which are from the same donor to ...
[摘要] 细胞粘附保证了相邻细胞之间的紧密接触，这对于细胞分离以及不同组织的形态和功能分化是必要的。显然，存在使相同类型的细胞优先彼此粘附的细胞 - 细胞识别系统。同型细胞粘附在介导一系列生理过程例如细胞存活，迁移和血管重塑中特别重要。因此，在本研究中，我们选择两个来自相同供体的内皮祖细胞群体，以研究小分子化合物Icariin对同型细胞粘附的可能影响。许多血管生成因子可以破坏细胞间连接的组织，导致内皮屏障开放。在本研究中，我们观察到Icariin治疗减少EPC中VE-钙粘蛋白表达的水平，表明细胞 - 细胞粘附减少 - 证明Icariin的促血管生成作用。总之，所观察到的EPCs的同型粘附的丧失可能有助于由Icariin施加的增强的血管生成作用。
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| Hedgehog (Hh) Reporter Activity Assay
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Author:
Date:
2014-07-20
[Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Hh ligands or Hh pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Hh pathway responses. Canonical Hh signaling activity transcriptionally induces Hh target genes that contain consensus Gli binding element. Hh-responsive cells transiently or stably expressing luciferase protein under the regulation of the Gli promoter element can be used to report stimulus-dependent Hh-pathway activity.
[摘要] 该方案用于测试候选细胞系/细胞系对Hh配体或Hh通路激动剂刺激的反应。 该方案还可以适于筛选含有增加或降低Hh通路反应的活性的小分子文库或生物制剂。 规范Hh信号活动转录诱导含有共有Gli结合元件的Hh靶基因。 在Gli启动子元件的调节下瞬时或稳定表达荧光素酶蛋白的Hh反应性细胞可用于报告刺激依赖性Hh-途径活性。
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| Wnt Reporter Activity Assay
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Author:
Date:
2014-07-20
[Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Wnt ligands or Wnt pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Wnt pathway responses. Canonical Wnt signaling activity transcriptionally induces Wnt target genes that contain concensus TCF/LEF binding element. Wnt pathway activity responsive cells transiently or stably expressing luciferase proteins under the TCF/LEF promoter element can be used to report stimulus-dependent Wnt-pathway activity. ...
[摘要] 该方案用于测试候选细胞系/细胞系对Wnt配体或Wnt通路激动剂刺激的反应。 该方案还可以适于筛选含有增加或降低Wnt途径反应的活性的小分子文库或生物制品。 规范Wnt信号活动转录诱导含有概括TCF/LEF结合元件的Wnt靶基因。 在TCF/LEF启动子元件下瞬时或稳定表达荧光素酶蛋白的Wnt途径活性应答性细胞可用于报告刺激依赖性Wnt通路活性。 我们从Addgene获得TopFlash（TCL/LEF-Firefly萤光素酶）构建体。
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