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ZebaTM Micro Spin Desalting Columns

Zeba TM微量离心脱盐柱

公司名称: Thermo Fisher Scientific
产品编号: 89877
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Protein-ligand Binding Assay by Liquid Chromatography-Mass Spectrometry
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2011-08-20
[Abstract]  Protein-small molecule binding coefficients are determined by quantitative LC-MS in this method. Traditional biochemical plot is consequently used to set up the binding curve between a known protein and a small molecule (<1000 da) to determine the affinity constant (kd) and the stoichiometry (bmax). da)="" to="" determine="" the="" affinity="" constant="" (kd)="" and="" the="" stoichiometry=""> [摘要]  本篇主要通过液相色谱-质谱法测定蛋白质与小分子物质的结合系数。传统的生物化学方法主要是依此利用已知蛋白质与一小分子(<1000 da)建立结合曲线来测定亲和常数 (kd) 和 化学计量 (bmax)。 da)建立结合曲线来测定亲和常数="" (kd)="" 和="" 化学计量="">

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