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BenchMarkTM His-tagged Protein Standard

BenchMark TM His标签蛋白标准

公司名称: Thermo Fisher Scientific
产品编号: LC5606
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In vitro Assay of the Glycosyltransferase Activity of a Heterologously Expressed Plant Protein
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2014-11-05
[Abstract]  Glycosyltransferases are carbohydrate active enzymes containing catalytic modules involved in catalysing the biosynthesis of glycosidic bonds in oligo- and polysaccharides and glycoconjugates. One of the most comprehensive collections of Carbohydrate Active enZYmes is the CAZy database (http://www.cazy.org) comprising 120,000 glycosyltransferases allocated to 96 families based mainly on sequence homologies of their conserved and catalytically active domains (Cantarel et al., 2009). Interestingly, the glycosyltransferase activities of ... [摘要]  糖基转移酶是含有催化模块的碳水化合物活性酶,其涉及催化寡糖和多糖和糖缀合物中糖苷键的生物合成。 Carbohydrate Active enZYmes最全面的集合之一是CAZy数据库( http://www.cazy.org ) ),其包含分配给96个家族的120,000个糖基转移酶,主要基于其保守和催化活性结构域的序列同源性(Cantarel等人,2009)。有趣的是,仅约1.6%的这些蛋白质的糖基转移酶活性已经通过实验表征(Lombard等人,2014)。近年来,已显示许多家族的膜结合糖基转移酶在植物细胞壁多糖的生物合成中起关键作用(Doblin等人,2010; Scheller和Ulvskov,2010 ; ...

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