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Author:
Date:
2016-04-05
[Abstract] Somatic chromosomes are usually studied from the root tip cells of the plants for cytological investigations. In dioecious plant species like Coccinia grandis, it is very difficult to get meristematic root tip cells from the mature plants of the respective sex forms. In this report, young leaves of the respective sexual phenotypes were used as tissue samples for mitotic chromosome analysis. For meiotic preparation, flower buds of appropriate size were selected for chromosomal studies. Following protocols could be effectively used for routine chromosome preparations in other plant ...
[摘要] 通常从植物的根尖细胞研究体细胞染色体用于细胞学研究。 在雌雄异种植物如Coccinia grandis中，很难从相应性别形式的成熟植物中获得分生组织根尖细胞。 在本报告中，各个性表型的幼叶用作有丝分裂染色体分析的组织样品。 对于减数分裂制备，选择适当大小的花芽用于染色体研究。 以下方案也可以有效地用于其他植物物种中的常规染色体制备。
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