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Author:
Date:
2014-12-20
[Abstract] The fluorescence-linked antigen quantification (FLAQ) assay allows a fast quantification of HIV-1 p24Gag antigen. Viral supernatant are lysed and incubated with polystyrene microspheres coated with polyclonal antibodies against HIV-1 p24Gag and detector antibodies conjugated to fluorochromes (Figure 1). After washes, the fluorescence of microspheres is measured by flow cytometry and reflects the abundance of the antigen in the lysate. The speed, simplicity, and wide dynamic range of the FLAQ assay are optimum for many applications performed in HIV-1 research ...
[摘要] 荧光连接的抗原定量（FLAQ）测定允许HIV-1p24Gag抗原的快速定量。 将病毒上清液裂解并与涂覆有针对HIV-1p24 Gag的多克隆抗体的聚苯乙烯微球和与荧光染料缀合的检测抗体（图1）温育。 洗涤后，通过流式细胞术测量微球的荧光，并反映裂解物中抗原的丰度。 FLAQ测定的速度，简单性和宽动态范围对于在HIV-1研究实验室中进行的许多应用是最佳的。
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