| Isolation of CNS-infiltrating and Resident Microglial Cells
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Author:
Date:
2015-01-20
[Abstract] Variety of immunological and biochemical studies associated with infection or inflammation in the central nervous system (CNS) utilize CNS-resident and/or infiltrating cells which were isolated from the CNS of naïve and affected mice in order to investigate the underlying mechanisms and the potential roles of the cell populations. Mechanical and enzyme-based single cell preparations of CNS cells are subjected to a density gradient to obtain functional single cells. In combination with cell-specific biomarkers, the function and/or status of resident microglia and infiltrating lymphocytes, ...
[摘要] 与中枢神经系统（CNS）中的感染或炎症相关的免疫学和生物化学研究的多样性利用从初始和受影响的小鼠的CNS中分离的CNS驻留和/或浸润细胞，以研究潜在的机制和潜在的作用 的细胞群体。 将CNS细胞的机械和基于酶的单细胞制剂进行密度梯度以获得功能性单细胞。 与细胞特异性生物标志物组合，可以表征驻留小胶质细胞和浸润淋巴细胞（包括B和T细胞以及巨噬细胞）的功能和/或状态。
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| Mitochondrial Isolation and Purification from Mouse Spinal Cord
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Author:
Date:
2013-11-05
[Abstract] Mitochondria are eukaryotic organelles that play a crucial role in several cellular processes, including energy production, β-oxidation of fatty acids and regulation of calcium homeostasis. In the last 20 years there has been a hightened interest in the study of mitochondria following the discoveries that mitochondria are central to the process of programmed cell death and that mitochondrial dysfunctions are implicated in numerous diseases including a wide range of neurological disorders such as Parkinson’s disease, Alzheimer’s disease, Huntington’s disease and amyotrophic lateral sclerosis. ...
[摘要] 线粒体是在几种细胞过程中发挥关键作用的真核细胞器，包括能量产生，脂肪酸的β-氧化和钙稳态的调节。在过去20年中，线粒体研究发现线粒体是程序性细胞死亡过程的核心，线粒体功能障碍涉及许多疾病，包括广泛的神经系统疾病如帕金森病，阿尔茨海默病，亨廷顿病和肌萎缩性侧索硬化。为了识别和研究与特定神经病学条件相关的线粒体功能的变化，线粒体通常从疾病中最受影响的中枢神经系统的隔室中分离出来。在这里，我们描述了从小鼠脊髓分离线粒体的方案，中枢神经系统的一个隔室在神经肌肉疾病如肌萎缩性侧索硬化症中受到显着影响。该方法依赖于差速离心法将线粒体与其他亚细胞隔离区分离。
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