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Open-Top Polyallomer Ultracentrifuge Tubes

开顶聚合物超速离心管

公司名称: Denville Scientific
产品编号: U5022
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Aβ Extraction from Murine Brain Homogenates
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2016-04-20
[Abstract]  This protocol details beta-amyloid (Aβ) extraction from transgenic murine brain homogenates. Specifically, mechanical homogenization of brain tissue and sequential extraction of both soluble and insoluble proteins are detailed. DEA extracts soluble proteins, such as Aβ isoforms and APP. Formic acid enables extraction of insoluble protein aggregates, such as Aβ isoforms associated with plaques. This procedure produces soluble and insoluble extracts that are amenable to analysis of Aβ species via western blotting and/or enzyme-linked immunosorbent assays (ELISAs), and these results help assess ... [摘要]  该方案详述了从转基因鼠脑匀浆中提取β-淀粉样蛋白(Aβ)。 具体来说,详细说明脑组织的机械均质化和可溶性和不溶性蛋白质的顺序提取。 DEA提取可溶性蛋白质,如Aβ异构体和APP。 甲酸能够提取不溶性蛋白质聚集体,例如与斑块相关的Aβ同种型。 该方法产生可溶性和不溶性提取物,其适于通过western印迹和/或酶联免疫吸附测定(ELISA)分析Aβ物质,并且这些结果有助于评估动物的淀粉样变性负担。

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